Differentiation of Dihydroxylated Vitamin D₃ Isomers Using Tandem Mass Spectrometry.

ABSTRACT: Vitamin D compounds are a group of secosteroids derived from cholesterol that are vital for maintaining bone health in humans. Recent studies have shown extraskeletal effects of vitamin D, involving vitamin D metabolites such as the dihydroxylated vitamin D₃ compounds 1,25-dihydroxyvitamin D₃ and 24,25-dihydroxyvitamin D₃. Differentiation and characterization of these isomers by mass spectrometry can be challenging due to the zero-mass difference and minor structural differences between them. The isomers usually require separation by liquid chromatography (LC) prior to mass spectrometry, which adds extra complexity to the analysis. Herein, we investigated and revisited the use of fragmentation methods such as collisional induced dissociation (CID), infrared

SUBMITTER: Haris A 

PROVIDER: S-EPMC9164238 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature