Highly Efficient Methods to Culture Mouse Cholangiocytes and Small Intestine Organoids.

ABSTRACT:

Background

Organoids, which enable disease modeling and drug screening closer to an in vivo environment, can be isolated and grown from organs such as the brain, small intestine, kidney, lungs, and liver. To facilitate the establishment of liver and small intestinal organoids, we developed efficient protocols for cholangiocytes and intestine crypts collecting and organoid culturing.

Methods

Cholangiocytes were collected from intrahepatic bile ducts, the gallbladder, and small intestine crypts by gravity settling and multistep centrifugation methods. The cells isolated were embedded with Matrigel and grew in three-dimensional spheroids in a suitable culture medium. The stability of organoid cells was assessed by subculture, cryopreservation, and thawing. RNA and DNA ex

SUBMITTER: Chen W 

PROVIDER: S-EPMC9164252 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature