The PRMT5-LSD1 axis confers Slug dual transcriptional activities and promotes breast cancer progression.

ABSTRACT:

Background

Downregulation of epithelial markers and upregulation of mesenchymal markers are the characteristics of the epithelial to mesenchymal transition (EMT) program, which provides the metastatic advantage of breast cancer. However, the mechanism underlying the switch of EMT markers remains poorly understood.

Methods

In this study, we used the affinity purification and mass spectrometry coupled approach to identify the interactome of Slug. CoIP, GST-pulldown, ChIP, Re-ChIP, qPCR and Immunoblot were used to investigate the underlying mechanism of Slug-PRMT5-LSD1 complex. The role of PRMT5 and LSD1 in breast cancer progression was evaluated both in vivo and in vitro.

Results

Here we found that the transcription factor Slug associates with PRMT5 and LSD1 in a comple

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9164399 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature