Bayesian analysis of dynamic phosphoproteomic data identifies protein kinases mediating GPCR responses.

ABSTRACT:

Background

A major goal in the discovery of cellular signaling networks is to identify regulated phosphorylation sites ("phosphosites") and map them to the responsible protein kinases. The V2 vasopressin receptor is a G-protein coupled receptor (GPCR) that is responsible for regulation of renal water excretion through control of aquaporin-2-mediated osmotic water transport in kidney collecting duct cells. Genome editing experiments have demonstrated that virtually all vasopressin-triggered phosphorylation changes are dependent on protein kinase A (PKA), but events downstream from PKA are still obscure.

Methods

Here, we used: 1) Tandem mass tag-based quantitative phosphoproteomics to experimentally track phosphorylation changes over time in native collecting ducts isolated fr

SUBMITTER: Leo KT 

PROVIDER: S-EPMC9164474 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature