Surprising Rigidity of Functionally Important Water Molecules Buried in the Lipid Headgroup Region.

ABSTRACT: Understanding water dynamics and structure is an important topic in biological systems. It is generally held in the literature that the interfacial water of hydrated phospholipids is highly mobile, in fast exchange with the bulk water ranging from the nano- to femtosecond timescale. Although nuclear magnetic resonance (NMR) is a powerful tool for structural and dynamic studies, direct probing of interfacial water in hydrated phospholipids is formidably challenging due to the extreme population difference between bulk and interfacial water. We developed a novel ¹⁷O solid-state NMR technique in combination with an ultra-high-field magnet (35.2 T) to directly probe the functionally important interfacial water. By selectively suppressing the dominant bulk water signal, we observed t

SUBMITTER: Zhang R 

PROVIDER: S-EPMC9165019 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature