Instantaneous synthesis and full characterization of organic-inorganic laccase-cobalt phosphate hybrid nanoflowers.

ABSTRACT: A novel approach termed the "concentrated method" was developed for the instant fabrication of laccase@Co₃(PO₄)₂•hybrid nanoflowers (HNFs). The constructed HNFs were obtained by optimizing the concentration of cobalt chloride and phosphate buffer to reach the highest activity recovery. The incorporation of 30 mM CoCl₂ and 160 mM phosphate buffer (pH 7.4) resulted in a fast anisotropic growth of the nanomaterials. The purposed method did not involve harsh conditions and prolonged incubation of precursors, as the most reported approaches for the synthesis of HNFs. The catalytic efficiency of the immobilized and free laccase was 460 and 400 M^-1S^-1, respectively. Also, the enzymatic activity of the prepared biocatalyst was 113%

SUBMITTER: Vojdanitalab K 

PROVIDER: S-EPMC9165545 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature