Mechanistic insights into actin force generation during vesicle formation from cryo-electron tomography.

ABSTRACT: Actin assembly provides force for a multitude of cellular processes. Compared to actin-assembly-based force production during cell migration, relatively little is understood about how actin assembly generates pulling forces for vesicle formation. Here, cryo-electron tomography identified actin filament number, organization, and orientation during clathrin-mediated endocytosis in human SK-MEL-2 cells, showing that force generation is robust despite variance in network organization. Actin dynamics simulations incorporating a measured branch angle indicate that sufficient force to drive membrane internalization is generated through polymerization and that assembly is triggered from ∼4 founding "mother" filaments, consistent with tomography data. Hip1R actin filament anchoring points are prese

SUBMITTER: Serwas D 

PROVIDER: S-EPMC9165722 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature