Pneumatic equiaxial compression device for mechanical manipulation of epithelial cell packing and physiology.

ABSTRACT: It is well established that mechanical cues, e.g., tensile- compressive- or shear forces, are important co-regulators of cell and tissue physiology. To understand the mechanistic effects these cues have on cells, technologies allowing precise mechanical manipulation of the studied cells are required. As the significance of cell density i.e., packing on cellular behavior is beginning to unravel, we sought to design an equiaxial cell compression device based on our previously published cell stretching system. We focused on improving the suitability for microscopy and the user-friendliness of the system. By introducing a hinge structure to the substrate stretch generating vacuum chamber, we managed to decrease the z-displacement of the cell culture substrate, thus reducing the focal plane dri

SUBMITTER: Peussa H 

PROVIDER: S-EPMC9165817 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature