Prediction of various blood group systems using Korean whole-genome sequencing data.

ABSTRACT:

Aims

This study established blood group analysis methods using whole-genome sequencing (WGS) data and conducted blood group analyses to determine the domestic allele frequency using public data from the Korean whole sequence analysis of the Korean Reference Genome Project conducted by the Korea Disease Control and Prevention Agency (KDCA).

Materials and methods

We analyzed the differences between the human reference sequences (hg19) and the conventional reference cDNA sequences of blood group genes using the Clustal Omega website, and established blood group analysis methods using WGS data for 41 genes, including 39 blood group genes involved in 36 blood group antigens, as well as the GATA1 and KLF1 genes, which are erythrocyte-specific transcription factor genes. Using CLC

SUBMITTER: Hyun J 

PROVIDER: S-EPMC9165885 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature