Quantification of SNAP-25 with mass spectrometry and Simoa: a method comparison in Alzheimer's disease.

ABSTRACT:

Background

Synaptic dysfunction and degeneration are central to Alzheimer's disease (AD) and have been found to correlate strongly with cognitive decline. Thus, studying cerebrospinal fluid (CSF) biomarkers reflecting synaptic degeneration, such as the presynaptic protein synaptosomal-associated protein 25 (SNAP-25), is of importance to better understand the AD pathophysiology.

Methods

We compared a newly developed Single molecule array (Simoa) immunoassay for SNAP-25 with an in-house immunoprecipitation mass spectrometry (IP-MS) method in a well-characterized clinical cohort (n = 70) consisting of cognitively unimpaired (CU) and cognitively impaired (CI) individuals with and without Aβ pathology (Aβ+ and Aβ-).

Results

A strong correlation (Spearman's rank correlation

SUBMITTER: Nilsson J 

PROVIDER: S-EPMC9166380 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature