Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism.

ABSTRACT:

Background

The methylotrophic budding yeast Pichia pastoris GS115 is a powerful expression system and hundreds of heterologous proteins have been successfully expressed in this strain. Recently, P. pastoris has also been exploited as an attractive cell factory for the production of high-value biochemicals due to Generally Recognized as Safe (GRAS) status and high growth rate of this yeast strain. However, appropriate regulation of metabolic flux distribution between cell growth and product biosynthesis is still a cumbersome task for achieving efficient biochemical production.

Results

In this study, P. pastoris was exploited for high inositol production using an effective dynamic regulation strategy. Through enhancing native inositol biosynthesis pathway, knocking out inosito

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC9166411 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature