The influence of Holliday junction sequence and dynamics on DNA crystal self-assembly.

ABSTRACT: The programmable synthesis of rationally engineered crystal architectures for the precise arrangement of molecular species is a foundational goal in nanotechnology, and DNA has become one of the most prominent molecules for the construction of these materials. In particular, branched DNA junctions have been used as the central building block for the assembly of 3D lattices. Here, crystallography is used to probe the effect of all 36 immobile Holliday junction sequences on self-assembling DNA crystals. Contrary to the established paradigm in the field, most junctions yield crystals, with some enhancing the resolution or resulting in unique crystal symmetries. Unexpectedly, even the sequence adjacent to the junction has a significant effect on the crystal assemblies. Six of the immobile junc

SUBMITTER: Simmons CR 

PROVIDER: S-EPMC9166708 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature