Structures of a mammalian TRPM8 in closed state.

ABSTRACT: Transient receptor potential melastatin 8 (TRPM8) channel is a Ca²⁺-permeable non-selective cation channel that acts as the primary cold sensor in humans. TRPM8 is also activated by ligands such as menthol, icilin, and phosphatidylinositol 4,5-bisphosphate (PIP₂), and desensitized by Ca²⁺. Here we have determined electron cryo-microscopy structures of mouse TRPM8 in the absence of ligand, and in the presence of Ca²⁺ and icilin at 2.5-3.2 Å resolution. The ligand-free state TRPM8 structure represents the full-length structure of mammalian TRPM8 channels with a canonical S4-S5 linker and the clearly resolved selectivity filter and outer pore loop. TRPM8 has a short but wide selectivity filter which may account for its permeability to hydrated Ca

SUBMITTER: Zhao C 

PROVIDER: S-EPMC9166780 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature