Dataset Information

Minority report: small-scale metagenomic analysis of the non-bacterial kitchen sponge microbiota.

ABSTRACT: Kitchen sponges are particularly well known to harbor a high number and diversity of bacteria, including pathogens. Viruses, archaea, and eukaryotes in kitchen sponges, however, have not been examined in detail so far. To increase knowledge on the non-bacterial kitchen sponge microbiota and its potential hygienic relevance, we investigated five used kitchen sponges by means of metagenomic shot-gun sequencing. Viral particles were sought to be enriched by a filter step during DNA extraction from the sponges. Data analysis revealed that ~ 2% of the sequences could be assigned to non-bacterial taxa. Each sponge harbored different virus (phage) species, while the present archaea were predominantly affiliated with halophilic taxa. Among the eukaryotic taxa, besides harmless algae, or amoebas, mainly DNA from food-left-overs was found. The presented work offers new insights into the complex microbiota of used kitchen sponges and contributes to a better understanding of their hygienic relevance.

SUBMITTER: Brandau L

PROVIDER: S-EPMC9167186 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Minority report: small-scale metagenomic analysis of the non-bacterial kitchen sponge microbiota.

Brandau Lena L Jacksch Susanne S Weis Severin S Schnell Sylvia S Egert Markus M

Archives of microbiology 20220604 7

Kitchen sponges are particularly well known to harbor a high number and diversity of bacteria, including pathogens. Viruses, archaea, and eukaryotes in kitchen sponges, however, have not been examined in detail so far. To increase knowledge on the non-bacterial kitchen sponge microbiota and its potential hygienic relevance, we investigated five used kitchen sponges by means of metagenomic shot-gun sequencing. Viral particles were sought to be enriched by a filter step during DNA extraction from ...[more]

PMID: 35661258

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Dataset Information

Minority report: small-scale metagenomic analysis of the non-bacterial kitchen sponge microbiota.

Publications

Minority report: small-scale metagenomic analysis of the non-bacterial kitchen sponge microbiota.

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