Persistent HIV transcription and variable antiretroviral drug penetration in lymph nodes during plasma viral suppression.

ABSTRACT:

Objective

The ability of antiretroviral drugs to penetrate and suppress viral replication in tissue reservoir sites is critical for HIV remission. We evaluated antiretroviral concentrations in lymph nodes and their impact on HIV transcription.

Methods

Participants of the RV254/SEARCH010 Acute HIV Infection Cohort in Thailand were enrolled. Group 1 (n  = 6) initiated and continued antiretrovirals with two nucleoside reverse transcriptase inhibitors (NRTIs), dolutegravir (DTG) and mar- aviroc (MVC). Group 2 (n = 12) initiated antiretrovirals with two NRTIs as well as efavirenz and were switched to two NRTIs as well as DTG. Antiretroviral concentrations were measured by mass spectroscopy. HIV RNA+ and DNA+ cells were measured by in-situ hybridization.

Results

All partici

SUBMITTER: Fletcher CV 

PROVIDER: S-EPMC9167254 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature