Genome-wide specificity of plant genome editing by both CRISPR-Cas9 and TALEN.

ABSTRACT: CRISPR and TALENs are efficient systems for gene editing in many organisms including plants. In many cases the CRISPR-Cas or TALEN modules are expressed in the plant cell only transiently. Theoretically, transient expression of the editing modules should limit unexpected effects compared to stable transformation. However, very few studies have measured the off-target and unpredicted effects of editing strategies on the plant genome, and none of them have compared these two major editing systems. We conducted, in Physcomitrium patens, a comprehensive genome-wide investigation of off-target mutations using either a CRISPR-Cas9 or a TALEN strategy. We observed a similar number of differences for the two editing strategies compared to control non-transfected plants, with an average of 8.25 SNV

SUBMITTER: Bessoltane N 

PROVIDER: S-EPMC9167288 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature