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Effect of Nosema ceranae infection and season on the gut bacteriome composition of the European honeybee (Apis mellifera).


ABSTRACT: Nosema ceranae is an intracellular parasite that infects honeybees' gut altering the digestive functions; therefore, it has the potential of affecting the composition of the gut microbiome. In this work, individual bees of known age were sampled both in spring and autumn, and their digestive tracts were assessed for N. ceranae infection. Intestinal microbiome was assessed by sequencing the bacterial 16S rRNA gene in two different gut sections, the anterior section (AS; midgut and a half of ileum) and the posterior section (PS; second half of ileum and rectum). A preliminary analysis with a first batch of samples (n = 42) showed that AS samples had a higher potential to discriminate between infected and non-infected bees than PS samples. As a consequence, AS samples were selected for subseq

SUBMITTER: Jabal-Uriel C 

PROVIDER: S-EPMC9167302 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature