The effect of hemolysis on quality control metrics for noninvasive prenatal testing.

ABSTRACT:

Background

Noninvasive prenatal testing (NIPT) is the testing of blood samples from pregnant women to screen for fetal risk of chromosomal disorders. Even though in vitro hemolysis of blood specimens is common in clinical laboratories, its influence on NIPT has not been well investigated.

Methods

Peripheral blood samples were collected from 205 pregnant women and categorized according to the concentration of free hemoglobin in the plasma. After performing NIPT using massively parallel sequencing, the quality control metrics were analyzed and compared with samples that did not undergo hemolysis or samples redrawn from the same women.

Results

The specimens were divided into four groups based on the concentration of free hemoglobin: Group I (0-1 g/L, n = 53), Group II (1

SUBMITTER: Guo Y 

PROVIDER: S-EPMC9167518 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature