5-HT7 receptors expressed in the mouse parafacial region are not required for respiratory chemosensitivity.

ABSTRACT: A brainstem homeostatic system senses CO₂ /H⁺ to regulate ventilation, blood gases and acid-base balance. Neurons of the retrotrapezoid nucleus (RTN) and medullary raphe are both implicated in this mechanism as respiratory chemosensors, but recent pharmacological work suggested that the CO₂ /H⁺ sensitivity of RTN neurons is mediated indirectly, by raphe-derived serotonin acting on 5-HT7 receptors. To investigate this further, we characterized Htr7 transcript expression in phenotypically identified RTN neurons using multiplex single cell qRT-PCR and RNAscope. Although present in multiple neurons in the parafacial region of the ventrolateral medulla, Htr7 expression was undetectable in most RTN neurons (Nmb⁺ /Phox2b⁺ ) concent

SUBMITTER: Shi Y 

PROVIDER: S-EPMC9167793 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature