The secretome of irradiated peripheral blood mononuclear cells attenuates activation of mast cells and basophils.

ABSTRACT:

Background

IgE-mediated hypersensitivity is becoming increasingly prevalent and activation of mast cells and basophils represent key events in the pathophysiology of allergy. We have previously reported that the secretome of γ-irradiated peripheral blood mononuclear cells (PBMCsec) exerts beneficial anti-inflammatory effects. Yet, its ability to alleviate allergic symptoms has not been investigated so far.

Methods

Several experimental in vitro and in vivo models have been used in this basic research study. A murine ear swelling model was used to study the effects of PBMCsec on 48/80-induced mast cell degranulation in vivo. The transcriptional profile of murine mast cells was analysed by single cell RNA sequencing (scRNAseq). Mast cell activation was studied in vitro using pr

SUBMITTER: Laggner M 

PROVIDER: S-EPMC9168057 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature