Revealing the Critical Regulators of Modulated Smooth Muscle Cells in Atherosclerosis in Mice.

ABSTRACT: Background: There are still residual risks for atherosclerosis (AS)-associated cardiovascular diseases to be resolved. Considering the vital role of phenotypic switching of smooth muscle cells (SMCs) in AS, especially in calcification, targeting SMC phenotypic modulation holds great promise for clinical implications. Methods: To perform an unbiased and systematic analysis of the molecular regulatory mechanism of phenotypic switching of SMCs during AS in mice, we searched and included several publicly available single-cell datasets from the GEO database, resulting in an inclusion of more than 80,000 cells. Algorithms implemented in the Seurat package were used for cell clustering and cell atlas depiction. The pySCENIC and SCENIC packages were used to identify master regulators

SUBMITTER: Zhou W 

PROVIDER: S-EPMC9168464 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature