High-Pressure Microfluidics for Ultra-Fast Microbial Phenotyping

ABSTRACT: Here, we present a novel methodology based on high-pressure microfluidics to rapidly perform temperature-based phenotyping of microbial strains from deep-sea environments. The main advantage concerns the multiple on-chip temperature conditions that can be achieved in a single experiment at pressures representative of the deep-sea, overcoming the conventional limitations of large-scale batch metal reactors to conduct fast screening investigations. We monitored the growth of the model strain Thermococcus barophilus over 40 temperature and pressure conditions, without any decompression, in only 1 week, whereas it takes weeks or months with conventional approaches. The results are later compared with data from the literature. An additional example is also shown for a hydrogenotrophic methanoge

SUBMITTER: Cario A 

PROVIDER: S-EPMC9168469 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature