Natural communities of Achromatium oxaliferum comprise genetically, morphologically, and ecologically distinct subpopulations.

ABSTRACT: The diversity and ecology of natural communities of the uncultivated bacterium Achromatium oxaliferum were studied by use of culture-independent approaches. 16S rRNA gene sequences were PCR amplified from DNA extracted from highly purified preparations of cells that were morphologically identified as A. oxaliferum present in freshwater sediments from three locations in northern England (Rydal Water, Jenny Dam, Hell Kettles). Cloning and sequence analysis of the PCR-amplified 16S rRNA genes revealed that multiple related but divergent sequences were routinely obtained from the A. oxaliferum communities present in all the sediments examined. Whole-cell in situ hybridization with combinations of fluorescence-labelled oligonucleotide probes revealed that the divergent sequences recovered from

SUBMITTER: Gray ND 

PROVIDER: S-EPMC91685 | biostudies-literature | 1999 Nov

REPOSITORIES: biostudies-literature