Matrix Inversion and Subset Selection (MISS): A pipeline for mapping of diverse cell types across the murine brain.

ABSTRACT: The advent of increasingly sophisticated imaging platforms has allowed for the visualization of the murine nervous system at single-cell resolution. However, current experimental approaches have not yet produced whole-brain maps of a comprehensive set of neuronal and nonneuronal types that approaches the cellular diversity of the mammalian cortex. Here, we aim to fill in this gap in knowledge with an open-source computational pipeline, Matrix Inversion and Subset Selection (MISS), that can infer quantitatively validated distributions of diverse collections of neural cell types at 200-μm resolution using a combination of single-cell RNA sequencing (RNAseq) and in situ hybridization datasets. We rigorously demonstrate the accuracy of MISS against literature expectations. Importantly, we show

SUBMITTER: Mezias C 

PROVIDER: S-EPMC9168512 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature