Genomics of Diversification of Pseudomonas aeruginosa in Cystic Fibrosis Lung-like Conditions.

ABSTRACT: Pseudomonas aeruginosa is among the most problematic opportunistic pathogens for adults with cystic fibrosis (CF), causing repeated and resilient infections in the lung and surrounding airways. Evidence suggests that long-term infections are associated with diversification into specialized types but the underlying cause of that diversification and the effect it has on the persistence of infections remains poorly understood. Here, we use evolve-and-resequence experiments to investigate the genetic changes accompanying rapid, de novo phenotypic diversification in lab environments designed to mimic two aspects of human lung ecology: spatial structure and complex nutritional content. After ∼220 generations of evolution, we find extensive genetic variation present in all environments, including

SUBMITTER: Schick A 

PROVIDER: S-EPMC9168666 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature