Specific Guanosines in the HIV-2 Leader RNA are Essential for Efficient Viral Genome Packaging.

ABSTRACT: HIV-2, a human pathogen that causes acquired immunodeficiency syndrome, is distinct from the more prevalent HIV-1 in several features including its evolutionary history and certain aspects of viral replication. Like other retroviruses, HIV-2 packages two copies of full-length viral RNA during virus assembly and efficient genome encapsidation is mediated by the viral protein Gag. We sought to define cis-acting elements in the HIV-2 genome that are important for the encapsidation of full-length RNA into viral particles. Based on previous studies of murine leukemia virus and HIV-1, we hypothesized that unpaired guanosines in the 5' untranslated region (UTR) play an important role in Gag:RNA interactions leading to genome packaging. To test our hypothesis, we targeted 18 guanosines located in

SUBMITTER: Umunnakwe CN 

PROVIDER: S-EPMC9168803 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature