Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer.

ABSTRACT:

Background

Pancreatic cancer is a highly malignant tumor of the digestive system.

Objective

Exosomal circular RNA can be used as a biomarker for the early diagnosis of pancreatic cancer.

Methods

The expression of various differentially expressed circRNAs in pancreatic cancer tissues was analyzed by gene chip, exosome expression was verified by electron microscopy and Western blotting, and the expression of exosomal circRNA in pancreatic cancer cells, tissues, and plasma were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Results

Compared with healthy controls, hsa_circ_0006220 and hsa_circ_0001666 were highly expressed in exosomes in the plasma of pancreatic cancer patients. The AUC values were 0.7817 for hsa_circ_0006220,

SUBMITTER: Hong L 

PROVIDER: S-EPMC9169197 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature