Impedance-Based Phenotypic Readout of Transporter Function: A Case for Glutamate Transporters.

ABSTRACT: Excitatory amino acid transporters (EAAT/SLC1) mediate Na⁺-dependent uptake of extracellular glutamate and are potential drug targets for neurological disorders. Conventional methods to assess glutamate transport in vitro are based on radiolabels, fluorescent dyes or electrophysiology, which potentially compromise the cell's physiology and are generally less suited for primary drug screens. Here, we describe a novel label-free method to assess human EAAT function in living cells, i.e., without the use of chemical modifications to the substrate or cellular environment. In adherent HEK293 cells overexpressing EAAT1, stimulation with glutamate or aspartate induced cell spreading, which was detected in real-time using an impedance-based biosensor. This change in cell morpholo

SUBMITTER: Sijben HJ 

PROVIDER: S-EPMC9169222 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature