Generation of functional lungs via conditional blastocyst complementation using pluripotent stem cells.

ABSTRACT: Millions of people worldwide with incurable end-stage lung disease die because of inadequate treatment options and limited availability of donor organs for lung transplantation¹. Current bioengineering strategies to regenerate the lung have not been able to replicate its extraordinary cellular diversity and complex three-dimensional arrangement, which are indispensable for life-sustaining gas exchange^2,3. Here we report the successful generation of functional lungs in mice through a conditional blastocyst complementation (CBC) approach that vacates a specific niche in chimeric hosts and allows for initiation of organogenesis by donor mouse pluripotent stem cells (PSCs). We show that wild-type donor PSCs rescued lung formation in genetically defective recipient mouse e

SUBMITTER: Mori M 

PROVIDER: S-EPMC9169232 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature