Separation of Heterotrophic Microalgae Crypthecodinium cohnii by Dielectrophoresis.

ABSTRACT: Microalgae constitute an abundant source of poly-unsaturated fatty acids which are applied in various biotechnological fields such as pharmaceuticals and food supplement. Separating microalgae cells with respect to their lipid content would establish a relevant at-line analytical technique. The present study demonstrates an electrical approach for the separation of the lipid-producing microalgae Crypthecodinium cohnii using the effect of dielectrophoresis (DEP) in a microfluidic flow cell. Microalgae were cultivated for 8 days, while cell growth was characterized by optical density, dry cell weight, glucose concentration and lipid content via fluorescence microscopy. The size distribution of cells during cultivation was thoroughly investigated, since the DEP force scal

SUBMITTER: Birkholz M 

PROVIDER: S-EPMC9169251 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature