NODeJ: an ImageJ plugin for 3D segmentation of nuclear objects.

ABSTRACT:

Background

The three-dimensional nuclear arrangement of chromatin impacts many cellular processes operating at the DNA level in animal and plant systems. Chromatin organization is a dynamic process that can be affected by biotic and abiotic stresses. Three-dimensional imaging technology allows to follow these dynamic changes, but only a few semi-automated processing methods currently exist for quantitative analysis of the 3D chromatin organization.

Results

We present an automated method, Nuclear Object DetectionJ (NODeJ), developed as an imageJ plugin. This program segments and analyzes high intensity domains in nuclei from 3D images. NODeJ performs a Laplacian convolution on the mask of a nucleus to enhance the contrast of intra-nuclear objects and allow their detection. We

SUBMITTER: Dubos T 

PROVIDER: S-EPMC9169307 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature