Inactivation of porcine epidemic diarrhea virus with electron beam irradiation under cold chain conditions.

ABSTRACT: The many instances of COVID-19 outbreaks suggest that cold chains are a possible route for the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, owing to the low temperatures of cold chains, which are normally below 0 °C, there are limited options for virus inactivation. Here, high-energy electron beam (E-beam) irradiation was used to inactivate porcine epidemic diarrhea virus (PEDV) under simulated cold chain conditions. This coronavirus was used as a surrogate for SARS-CoV-2. The possible mechanism by which high-energy E-beam irradiation inactivates PEDV was also explored. An irradiation dose of 10 kGy reduced the PEDV infectious viral titer by 1.68-1.76 log₁₀TCID 50/100 μ L in the cold chain environment, suggesting that greater than 98.1% of PED

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9169434 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature