Serum Protein Adsorption Modulates the Toxicity of Highly Positively Charged Hydrogel Surfaces.

ABSTRACT: Hydrogels formed from peptide self-assembly are a class of materials that are being explored for their utility in tissue engineering, drug and cell delivery, two- and three-dimensional cell culture, and as adjuvants in surgical procedures. Most self-assembled peptide gels can be syringe-injected in vivo to facilitate the local delivery of payloads, including cells, directly to the targeted tissue. Herein, we report that highly positively charged peptide gels are inherently toxic to cells, which would seem to limit their utility. However, adding media containing fetal bovine serum, a common culture supplement, directly transforms these toxic gels into cytocompatible materials capable of sustaining cell viability even in the absence of added nutrients. Multistage mass spectrometry showed tha

SUBMITTER: Yamada Y 

PROVIDER: S-EPMC9169696 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature