Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy.

ABSTRACT: Cell mass and chemical composition are important aggregate cellular properties that are especially relevant to physiological processes, such as growth control and tissue homeostasis. Despite their importance, it has been difficult to measure these features quantitatively at the individual cell level in intact tissue. Here, we introduce normalized Raman imaging (NoRI), a stimulated Raman scattering (SRS) microscopy method that provides the local concentrations of protein, lipid, and water from live or fixed tissue samples with high spatial resolution. Using NoRI, we demonstrate that protein, lipid, and water concentrations at the single cell are maintained in a tight range in cells under the same physiological conditions and are altered in different physiological states, such as cell cycle

SUBMITTER: Oh S 

PROVIDER: S-EPMC9169924 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature