Vitamin D-mediated effects on airway innate immunity in vitro.

ABSTRACT:

Introduction

Vitamin D supplementation has been suggested to enhance immunity during respiratory infection season. We tested the effect of active vitamin D (calcitriol) supplementation on key airway innate immune mechanisms in vitro.

Methods

Primary human airway epithelial cells (hAECs) grown at the air liquid interface were supplemented with 10-7 M calcitriol for 24 hours (or a time course) and their antimicrobial airway surface liquid (ASL) was tested for pH, viscoscity, and antibacterial and antiviral properties. We also tested hAEC ciliary beat frequency (CBF). Next, we assessed alterations to hAEC gene expression using RNA sequencing, and based on results, we measured neutrophil migration across hAECs.

Results

Calcitriol supplementation enhanced ASL bacterial kil

SUBMITTER: Stapleton EM 

PROVIDER: S-EPMC9170100 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature