Evaluation of real-time NASBA assay for the detection of SARS-CoV-2 compared with real-time PCR.

ABSTRACT:

Purpose

In January 2020, the COVID-19 pandemic started and has severely affected all countries around the world. The clinical symptoms alone are not sufficient for a proper diagnosis. Thus, molecular tests are required. Various institutes and researchers developed real-time PCR-based methods for the detection of the virus. However, the method needs expensive equipment. In the present study, we developed a real-time NASBA assay for the detection of SARS-CoV-2.

Methods

Primers and molecular beacon probes for RdRp and N genes were designed. In silico analysis showed that primers and the probes were specific for SARS-CoV-2. The standard samples with known copy numbers of the virus were tested using the NASBA assay and an FDA-approved real-time PCR kit. A series of standard sampl

SUBMITTER: Kia V 

PROVIDER: S-EPMC9170118 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature