Cell Surface Engineering Using DNAzymes: Metal Ion Mediated Control of Cell-Cell Interactions.

ABSTRACT: Regulating cell-cell interactions and cell behaviors via cell surface engineering is of significance for biological research such as cell fate control and cell therapy. While extensive efforts have been made to induce cell-cell assembly via various cell surface modifications triggered by macromolecules or organic metabolites, controllable cell-cell interactions that include both assembly and disassembly triggered by metal ions remain a challenge. Herein, we report a strategy based on DNAzymes to realize controllable cell-cell interactions, triggered by metal ions. The metal-dependent DNAzyme-based cleavage can effectively manipulate cell behaviors, including cell-cell conjunctions and disaggregation. Using a Zn²⁺-specific DNAzyme, a Mg²⁺-specific DNAzyme, and their re

SUBMITTER: Qian RC 

PROVIDER: S-EPMC9170360 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature