Shotgun metagenomics of soil invertebrate communities reflects taxonomy, biomass, and reference genome properties.

ABSTRACT: Metagenomics - shotgun sequencing of all DNA fragments from a community DNA extract - is routinely used to describe the composition, structure, and function of microorganism communities. Advances in DNA sequencing and the availability of genome databases increasingly allow the use of shotgun metagenomics on eukaryotic communities. Metagenomics offers major advances in the recovery of biomass relationships in a sample, in comparison to taxonomic marker gene-based approaches (metabarcoding). However, little is known about the factors which influence metagenomics data from eukaryotic communities, such as differences among organism groups, the properties of reference genomes, and genome assemblies.We evaluated how shotgun metagenomics records composition and biomass in artificial soil inverteb

SUBMITTER: Schmidt A 

PROVIDER: S-EPMC9170594 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature