Histological and Top-Down Proteomic Analyses of the Visual Pathway in the Cuprizone Demyelination Model.

ABSTRACT: A change in visual perception is a frequent early symptom of multiple sclerosis (MS), the pathoaetiology of which remains unclear. Following a slow demyelination process caused by 12 weeks of low-dose (0.1%) cuprizone (CPZ) consumption, histology and proteomics were used to investigate components of the visual pathway in young adult mice. Histological investigation did not identify demyelination or gliosis in the optic tracts, pretectal nuclei, superior colliculi, lateral geniculate nuclei or visual cortices. However, top-down proteomic assessment of the optic nerve/tract revealed a significant change in the abundance of 34 spots in high-resolution two-dimensional (2D) gels. Subsequent liquid chromatography-tandem mass spectrometry (LC-TMS) analysis identified alterations in 75 proteoforms

SUBMITTER: Almuslehi MSM 

PROVIDER: S-EPMC9170674 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature