Mitochondrial reactive oxygen is critical for IL-12/IL-18-induced IFN-γ production by CD4⁺ T cells and is regulated by Fas/FasL signaling.

ABSTRACT: Mitochondrial activation and the production of mitochondrial reactive oxygen species (mROS) are crucial for CD4⁺ T cell responses and have a role in naïve cell signaling after TCR activation. However, little is known about mROS role in TCR-independent signaling and in recall responses. Here, we found that mROS are required for IL-12 plus IL-18-driven production of IFN-γ, an essential cytokine for inflammatory and autoimmune disease development. Compared to TCR stimulation, which induced similar levels of mROS in naïve and memory-like cells, IL-12/IL-18 showed faster and augmented mROS production in memory-like cells. mROS inhibition significantly downregulated IFN-γ and CD44 expression, suggesting a direct mROS effect on memory-like T cell function. The mechanism that promotes I

SUBMITTER: Rackov G 

PROVIDER: S-EPMC9170726 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature