Artificially sporulated Escherichia coli cells as a robust cell factory for interfacial biocatalysis.

ABSTRACT: The natural bacterial spores have inspired the development of artificial spores, through coating cells with protective materials, for durable whole-cell catalysis. Despite attractiveness, artificial spores developed to date are generally limited to a few microorganisms with their natural endogenous enzymes, and they have never been explored as a generic platform for widespread synthesis. Here, we report a general approach to designing artificial spores based on Escherichia coli cells with recombinant enzymes. The artificial spores are simply prepared by coating cells with polydopamine, which can withstand UV radiation, heating and organic solvents. Additionally, the protective coating enables living cells to stabilize aqueous-organic emulsions for efficient interfacial biocatalysis ranging

SUBMITTER: Sun Z 

PROVIDER: S-EPMC9170730 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature