Assessment of fluorescent protein candidates for multi-color flow cytometry analysis of Saccharomyces cerevisiae.

ABSTRACT: Transcription factor-based biosensors represent promising tools in the construction and evaluation of efficient cell factories for the sustainable production of fuels, chemicals and pharmaceuticals. They can notably be designed to follow the production of a target compound or to monitor key cellular properties, such as stress or starvation. In most cases, the biosensors are built with fluorescent protein (FP) genes as reporter genes because of the direct correlation between promoter activity and fluorescence level that can be measured using, for instance, flow cytometry or fluorometry. The expansion of available FPs offers the possibility of using several FPs - and biosensors - in parallel in one host, with simultaneous detection using multicolor flow cytometry. However, the technique is c

SUBMITTER: Perruca-Foncillas R 

PROVIDER: S-EPMC9171426 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature