RAR-Related Orphan Receptor: An Accelerated Preeclampsia Progression by Activating the JAK/STAT3 Pathway.

ABSTRACT:

Purpose

To investigate the effect and underlying mechanism of RAR related orphan receptor A (RORA) on preeclampsia (PE).

Materials and methods

Differentially expressed genes (DEGs) in four datasets were obtained by using the Venn diagram method. RORA mRNA and protein expressions were detected by qRT-PCR, western blot, and immunohistochemistry. HTR-8/SVneo cell viability, proliferation, invasion, migration, and angiogenesis were detected by CCK-8 assay, EdU assay, Transwell, wound healing assay, and tube formation assay, respectively. The concentration of Ang-1 in cells was assessed using available ELISA kit. Epithelial-mesenchymal transition, proliferation, and angiogenesis-related proteins were detected by western blot. GSEA analysis were performed for common DEGs, and the

SUBMITTER: Yu Y 

PROVIDER: S-EPMC9171667 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature