Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa.

ABSTRACT: Improving our understanding of host–microbe relationships in the gut requires the ability to both visualize and quantify the spatial organization of microbial communities in their native orientation with the host tissue. We developed a systematic procedure to quantify the three-dimensional (3D) spatial structure of the native mucosal microbiota in any part of the intestines with taxonomic and high spatial resolution. We performed a 3D biogeographical analysis of the microbiota of mouse cecal crypts at different stages of antibiotic exposure. By tracking eubacteria and four dominant bacterial taxa, we found that the colonization of crypts by native bacteria is a dynamic and spatially organized process. Ciprofloxacin treatment drastically reduced bacterial loads and eliminated Muribaculaceae

SUBMITTER: Mondragon-Palomino O 

PROVIDER: S-EPMC9171773 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature