Accurate Proteomewide Measurement of Methionine Oxidation in Aging Mouse Brains.

ABSTRACT: The oxidation of methionine has emerged as an important post-translational modification of proteins. A number of studies have suggested that the oxidation of methionines in select proteins can have diverse impacts on cell physiology, ranging from detrimental effects on protein stability to functional roles in cell signaling. Despite its importance, the large-scale investigation of methionine oxidation in a complex matrix, such as the cellular proteome, has been hampered by technical limitations. We report a methodology, methionine oxidation by blocking (MobB), that allows for accurate and precise quantification of low levels of methionine oxidation typically observed in vivo. To demonstrate the utility of this methodology, we analyzed the brain tissues of young (6 m.o.) and old (20 m.o.) m

SUBMITTER: Bettinger JQ 

PROVIDER: S-EPMC9171897 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature