Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens.

ABSTRACT:

Background

Immunoassay platforms that simultaneously detect malaria antigens including histidine-rich protein 2 (HRP2)/HRP3 and Plasmodium lactate dehydrogenase (pLDH), are useful epidemiological tools for rapid diagnostic test evaluation. This study presents the comparative evaluation of two multiplex platforms in identifying Plasmodium falciparum with presence or absence of HRP2/HRP3 expression as being indicative of hrp2/hrp3 deletions and other Plasmodium species. Moreover, correlation between the malaria antigen measurements performed at these platforms is assessed after calibrating with either assay standards or international standards and the cross-reactivity among Plasmodium species is examined.

Methods

A 77-member panel of specimens composed of the World Health Orga

SUBMITTER: Jang IK 

PROVIDER: S-EPMC9171962 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature