A microfluidic paper analytical device using capture aptamers for the detection of PfLDH in blood matrices.

ABSTRACT:

Background

The prevalence and death rate arising from malaria infection, and emergence of other diseases showing similar symptoms to malaria require the development of malaria-specific and sensitive devices for its diagnosis. To address this, the design and fabrication of low-cost, rapid, paper-based analytical devices (µPAD) using surface-immobilized aptamers to detect the presence of a recombinant malarial biomarker-Plasmodium falciparum lactate dehydrogenase (rPfLDH)-is reported in this study.

Methods

Test zones on paper surfaces were created by covalently immobilizing streptavidin to the paper, subsequently attaching biotinylated aptamers to streptavidin. Aptamers selectively bound rPfLDH. The measurement of captured rPfLDH enzyme activity served as the means of detectin

SUBMITTER: Ogunmolasuyi AM 

PROVIDER: S-EPMC9172074 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature