Hyperpolarized [5-¹³C,4,4-²H₂,5-¹⁵N]-L-glutamine provides a means of annotating in vivo metabolic utilization of glutamine.

ABSTRACT: Glutamine is consumed by rapidly proliferating cells and can provide the carbon and nitrogen required for growth through various metabolic pathways. However, delineating the metabolic fate of glutamine is challenging to interrogate in vivo. Hyperpolarized magnetic resonance, by providing high transient nuclear magnetic resonance signals, provides an approach to measure fast biochemical processes in vivo. Aminohydrolysis of glutamine at carbon-5 plays an important role in providing nitrogen and carbon for multiple pathways. Here, we provide a synthetic strategy for isotope-enriched forms of glutamine that prolongs glutamine-C5 relaxation times and thereby reveals in vivo reactions involving carbon-5. We investigate multiple enrichment states, finding [5-13C,4,4-2H2,5-15N]-L-glutamine to be

SUBMITTER: Eskandari R 

PROVIDER: S-EPMC9172133 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature