The quantum dot vs. organic dye conundrum for ratiometric FRET-based biosensors: which one would you chose?

ABSTRACT: Förster resonance energy transfer (FRET) is a widely used and ideal transduction modality for fluorescent based biosensors as it offers high signal to noise with a visibly detectable signal. While intense efforts are ongoing to improve the limit of detection and dynamic range of biosensors based on biomolecule optimization, the selection of and relative location of the dye remains understudied. Herein, we describe a combined experimental and computational study to systematically compare the nature of the dye, i.e., organic fluorophore (Cy5 or Texas Red) vs. inorganic nanoparticle (QD), and the position of the FRET donor or acceptor on the biomolecular components. Using a recently discovered transcription factor (TF)-deoxyribonucleic acid (DNA) biosensor for progesterone, we e

SUBMITTER: Grazon C 

PROVIDER: S-EPMC9172442 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature