Connectivity Mapping Using a Novel sv2a Loss-of-Function Zebrafish Epilepsy Model as a Powerful Strategy for Anti-epileptic Drug Discovery.

ABSTRACT: Synaptic vesicle glycoprotein 2A (SV2A) regulates action potential-dependent neurotransmitter release and is commonly known as the primary binding site of an approved anti-epileptic drug, levetiracetam. Although several rodent knockout models have demonstrated the importance of SV2A for functional neurotransmission, its precise physiological function and role in epilepsy pathophysiology remains to be elucidated. Here, we present a novel sv2a knockout model in zebrafish, a vertebrate with complementary advantages to rodents. We demonstrated that 6 days post fertilization homozygous sv2a^-/- mutant zebrafish larvae, but not sv2a ^+/- and sv2a^+/+ larvae, displayed locomotor hyperactivity and spontaneous epileptiform discharges, howeve

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9172968 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature